Staining technique

Application of

Gram staining 

Ziehl-Neelsen technique

Primary stain

Crystal violet

Carbol fuchsin

Mordant

Iodine

Heat

Decolorization

Acetone / Alcohal

Sulfuric acid

Counter stain

Safranin / Carbol fuchsin

Methylene blue

Result

Gram positive: Purple / Violet

Gram negative: Pink

Acid fast: Red

Non-acid fast: Blue

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