|
Application of |
Gram staining |
Ziehl-Neelsen technique |
|
Primary stain |
Crystal violet |
Carbol fuchsin |
|
Mordant |
Iodine |
Heat |
|
Decolorization |
Acetone / Alcohal |
Sulfuric acid |
|
Counter stain |
Safranin / Carbol fuchsin |
Methylene blue |
|
Result |
Gram positive: Purple / Violet Gram negative: Pink |
Acid fast: Red Non-acid fast: Blue |