Anti-Neutrophil Cytoplasmic Antibodies (ANCA)

Aspect

c-ANCA (Cytoplasmic)

p-ANCA (Perinuclear)

Target
Antigen

Proteinase 3 (PR3)

Myeloperoxidase (MPO)

Staining
Pattern

Cytoplasmic 

(diffuse granular staining)

Perinuclear 

(around the nucleus)

Associated 

Diseases

- Granulomatosis with 

polyangiitis (GPA)

- Microscopic polyangiitis 

(MPA)

- Eosinophilic granulomatosis 

with polyangiitis (EGPA)

- Ulcerative collitis

Clinical
Features

Upper and lower respiratory 

tract + renal involvement

Renal + systemic vasculitis 

symptoms

Diagnosis

ELISA for PR3 

antibodies

ELISA for MPO 

antibodies